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Why Chinese Hamster Ovary Cell use for antibody development ?

Chinese hamster

The Chinese hamster (Cricetulus griseus) is a small rodent in the genus Cricetulus of the subfamily Cricetidaet that originated in the deserts of northern China and Mongolia. They are distinguished by an uncommonly long tail in comparison to other hamsters, most of whose tails are stubby.

CHO cells have several advantages that make them a popular choice for bioproduction. They can adapt to grow in suspension or adherent cultures, allowing for scalable production. They have a high growth rate and can reach high cell densities, which is beneficial for large-scale manufacturing

Facts of Chinese Hamster

Chinese hamster is predominantly nocturnal creatures, although they may remain awake for short periods during the day in between their naps.re predominantly nocturnal creatures, with brief periods of wakefulness during the day sandwiched between their regular naps.

Chinese hamster
Chinese hamster

Chinese hamster set themselves apart from other hamster with their unusually long tails, unlike the stubby tails commonly seen in other breeds. While Chinese hamster predominantly exhibit nocturnal behavior.

Here are some facts about Chinese hamsters:

  • They are about 8-13 centimeters long and weigh 30-45 grams.
  • They have a grayish-black coat with a black dorsal stripe.
  • They have a long tail, about 2-3 centimeters long.
  • They are nocturnal animals.
  • They are omnivorous, but they prefer to eat seeds, fruits, and vegetables.
  • They are social animals groups of up to 10 .
  • They are pregnant for about 21 days and give birth to litters of 4-8 pups.
  • The pups are born blind and helpless and are weaned at about 3 weeks old.
  • They have a 2-3 years lifespan .

If you are considering getting a Chinese hamster, it is important to do your research and find a reputable breeder. You should also make sure that you have the proper cage and supplies before bringing your new pet home.

Here are some things to keep in mind when caring for a Chinese hamster:

  • The cage should be at least 20 gallons in size and have a solid bottom.
  • The cage should have a variety of toys and hiding places.
  • The hamster should have access to fresh water and food at all times.
  • The cage should be cleaned and disinfected regularly.
  • The hamster should be handled regularly to socialize it.

With proper care, Chinese hamsters can make good pets. They are relatively low-maintenance and can be quite entertaining to watch.

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Why use Chinese hamster ovary cell

Chinese hamster ovary (CHO) cells are an epithelial cell line derived from the ovary of the Chinese hamster. They are often used in biological and medical research and commercially in the production of recombinant therapeutic proteins.

Chinese hamster ovary cell
Chinese hamster ovary cell

CHO cells have a number of advantages that make them well-suited for these applications. They are easy to grow in large-scale cultures, have high viability, and are tolerant to variations in parameters such as oxygen levels, pH, temperature, and cell density. Additionally, CHO cells are able to produce proteins with complex glycosylations, post-translational modifications (PTMs) similar to those produced in humans.

Specific uses of Chinese hamster ovary cells include:

  • Genomic and chromosome studies: CHO cells are used to study the structure and function of chromosomes. They are also used to screen for chromosomal abnormalities, such as those that can be caused by exposure to radiation or chemicals.
  • Toxicity assays: CHO cells are used to assess the toxicity of new drugs and chemicals. They are also used to study the mechanisms of toxicity.
  • Nutrition and gene expression: CHO cells are used to study the effects of different nutrients on gene expression. They are also used to study the mechanisms by which genes are regulated.
  • Production of recombinant therapeutic proteins:┬áCHO cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutic proteins. These proteins include monoclonal antibodies, growth factors, and enzymes.

Overall, CHO cells are a versatile cell line with a wide range of applications in biological and medical research. They are particularly well-suited for the production of recombinant therapeutic proteins.

Additional facts about CHO cells:

  • They were first isolated in 1959 by George Gey.
  • The most commonly used CHO cell line is CHO-K1.
  • CHO cells are diploid, meaning they have two copies of each chromosome.
  • CHO cells can be grown in serum-free media, which makes them more cost-effective to produce.
  • CHO cells are not tumorigenic, meaning they do not form tumors when injected into animals.

I hope this helps! Let me know if you have any other questions.

How Chinese hamster Ovary Cell use for antibody development

Yes, Chinese hamster ovary (CHO) cells are very helpful for the production of antibodies. They are able to produce antibodies with complex glycosylation’s, post-translational modifications (PTMs) similar to those produced in humans. This is important because the glycosylation pattern of an antibody can affect its function and stability.

Ovary Cell use antibody development
Ovary Cell use antibody development

In addition, CHO cells are easy to grow in large-scale cultures, have high viability, and are tolerant to variations in parameters such as oxygen levels, pH, temperature, and cell density. This makes them well-suited for the industrial production of antibodies.

Over the past few decades, CHO cells have been used to produce a wide range of therapeutic antibodies, including Humira, Rituxan, and Herceptin. These antibodies have been used to treat a variety of diseases, including rheumatoid arthritis, cancer, and autoimmune disorders.

As a result of their versatility and efficiency, CHO cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutic proteins. They are a valuable tool for the development of new antibody-based therapies.

Examples of antibodies produced in Chinese Hamster Ovary cells:

  • Humira (adalimumab): This is a monoclonal antibody that is used to treat rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, ulcerative colitis, and Crohn’s disease.
  • Rituxan (rituximab): This is a monoclonal antibody that is used to treat non-Hodgkin’s lymphoma, chronic lymphocytic leukemia, and rheumatoid arthritis.
  • Herceptin (trastuzumab): This is a monoclonal antibody that is used to treat breast cancer.

These are just a few examples of the many antibodies that have been produced in CHO cells. As the field of antibody therapy continues to develop, CHO cells are likely to play an even more important role in the production of new and effective treatments.

CHO cells are considered a valuable tool in biotechnology due to their robustness, versatility, and compatibility with large-scale manufacturing processes. Their use has contributed significantly to the production of therapeutic proteins and other biopharmaceuticals that are crucial for treating various diseases.

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